HPLC METHOD DEVELOPMENT AND VALIDATION OF LERCANIDIPINE HCL AND ATENOLOL, CHARACTERIZATION OF ITS DEGRADANTS BY LC-MS/MS

Objective: An assay method was developed and validated for the simultaneous estimation of Lercanipine HCl atenolol using RP-HPLC. Methods: effective chromatographic separation achieved waters symmetry C18 column dimensions 150x4.6 mm, 3.5 μm, as a stationary phase. 0.1 percent ortho phosphoric acid acetonitrile in 50:50 v/v used mobile process with rate flow 1 ml/min UV detection carried out at 230 nm, respectively. Isocratic chromatography ambient temperature performed. Results: Lercanidipine were separated by running time around 8 min. 2.925 6.482 Respectively. By injecting norm six times, device suitability parameters studied outcomes well under acceptance criteria. The linearity analysis performed levels ranging from 10% to 150% R2 value found be 0.999. Conclusion: Assay validation marketed formulation within limit. Degradation tests conducted degradants characterized LC-MS/MS.